Determination of Chlorides in Biological Fluids by the Use of Adsorption Indicators the Use of Dichlorofluorescein for the Volumetric Microdetermination of Chlorides in Zinc Filtrates of Biological Fluids by Abraham Saifer and James Hughes

نویسندگان

  • ABRAHAM SAIFER
  • JAMES HUGHES
چکیده

This paper presents evidence that zinc filtrates (particularly the Hagedorn-Jensen (1923) filtrates) of urine (after treatment with hydrogen peroxide), whole blood, plasma, or serum are suitable for the accurate determination of chlorides with dichlorofluorescein as an indicator. The precision of this method for urine chlorides has been shown by comparison with established procedures such as the Caldwell-Moyer (1935) modificat.ion of the Volhard-Harvey (Harvey, 1910) method and the Van Slyke (1923-24) nitric acid digestion method for both normal and albuminous urines as recommended by Sendroy (1937). The method requires only one standard solution (0.02 N AgN03) and one indicator (0.05 per cent dichlorofluorescein in 50 per cent alcohol) for the determination of chlorides in urine, cerebrospinal fluids, blood serum, and plasma.

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تاریخ انتشار 2003